Journal: Heliyon

Article Title: The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF 165 and downregulating VEGF 165b .

doi: 10.1016/j.heliyon.2023.e18706

Figure Lengend Snippet: Fig. 3. YBX1 upregulated VEGF165 but downregulated VEGF165b in OS cells. A and B. Real-time PCR and Western blotting were used to verify the overexpression or knockdown of YBX1 in SaOS2 cells. The levels of VEGF165 and VEGF165b were also determined in those cells to confirm the effect of YBX1 on VEGF isoforms. C. A RIP assay was used to confirm the direct binding of YBX1 to VEGF mRNA. D. VEGF165 and VEGF165b levels in the culture supernatant of SaOS2 cells with YBX1 overexpression or knockdown were determined by ELISA.

Article Snippet: VEGF165b and panVEGF165 in the cell culture supernatant were measured by ELISA kits (R&D Systems, Minneapolis, MN, USA, cat. no. DY3045 for VEGF165b and cat. no. DVE00 for VEGF165) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Over Expression, Knockdown, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF 165 and downregulating VEGF 165b .

doi: 10.1016/j.heliyon.2023.e18706

Figure Lengend Snippet: Fig. 2. YBX1 was the underlying splicing factor regulating the formation of VEGF165 isoforms. A. Four splicing factors were obtained by overlapping the RNA-binding proteins targeting VEGF165 in the MEME and RBPDB datasets. B. Real-time PCR was used to determine the expression of four splicing factors in OS tissues and matched normal tissues of 18 OS patients. C. The correlation between VEGF165 and four splicing factors was analysed in OS tissues of 18 patients based on the real-time PCR results. D. The correlation between VEGF165b and four splicing factors was analysed in OS tissues of 18 patients based on the real-time PCR results.

Article Snippet: VEGF165b and panVEGF165 in the cell culture supernatant were measured by ELISA kits (R&D Systems, Minneapolis, MN, USA, cat. no. DY3045 for VEGF165b and cat. no. DVE00 for VEGF165) according to the manufacturer’s instructions.

Techniques: RNA Binding Assay, Real-time Polymerase Chain Reaction, Expressing

Journal: Heliyon

Article Title: The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF 165 and downregulating VEGF 165b .

doi: 10.1016/j.heliyon.2023.e18706

Figure Lengend Snippet: Fig. 3. YBX1 upregulated VEGF165 but downregulated VEGF165b in OS cells. A and B. Real-time PCR and Western blotting were used to verify the overexpression or knockdown of YBX1 in SaOS2 cells. The levels of VEGF165 and VEGF165b were also determined in those cells to confirm the effect of YBX1 on VEGF isoforms. C. A RIP assay was used to confirm the direct binding of YBX1 to VEGF mRNA. D. VEGF165 and VEGF165b levels in the culture supernatant of SaOS2 cells with YBX1 overexpression or knockdown were determined by ELISA.

Article Snippet: VEGF165b and panVEGF165 in the cell culture supernatant were measured by ELISA kits (R&D Systems, Minneapolis, MN, USA, cat. no. DY3045 for VEGF165b and cat. no. DVE00 for VEGF165) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Over Expression, Knockdown, Binding Assay, Enzyme-linked Immunosorbent Assay